کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8747073 | 1593421 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of high-resolution melting curve analysis to differentiate vaccine and wild type strains of grass carp reovirus genotype II
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Use of high-resolution melting curve analysis to differentiate vaccine and wild type strains of grass carp reovirus genotype II Use of high-resolution melting curve analysis to differentiate vaccine and wild type strains of grass carp reovirus genotype II](/preview/png/8747073.png)
چکیده انگلیسی
Grass carp reovirus (GCRV) is the causative agent of a hemorrhagic disease that causes severe economic losses in the grass carp (Ctenopharyngodon idella) farming industry in China. Discrimination between wild-type field and vaccine strains of GCRV is crucial for meaningful disease diagnosis and epidemiological investigation, yet current detection methods do not discriminate between these different viruses. This study exploited sequence differences between vaccine viruses and the virulent and field strains in the S6 gene that is present in all GCRV strains to develop a high resolution melting curve assay to differentiate between virus strains. The high resolution melting curve analysis was as analytically sensitive as real-time quantitative-Polymerase Chain Reaction (qPCR) detection and at least 10 times sensitive than the conventional PCR. This one-step assay will facilitate grass carp hemorrhagic disease outbreak responses and control.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 256, June 2018, Pages 111-115
Journal: Journal of Virological Methods - Volume 256, June 2018, Pages 111-115
نویسندگان
Yanmin Guo, Weiwei Zeng, Qing Wang, Yingying Wang, Yingying Li, Jiyuan Yin, Yan Ren, Cunbin Shi,