کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8747129 | 1593424 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3
ترجمه فارسی عنوان
تست آمپلیفایز ایزوترمال متصل به حلقه برای تشخیص سریع و بصری دایرو ویروس جدید خوک 3
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40â¯min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 253, March 2018, Pages 26-30
Journal: Journal of Virological Methods - Volume 253, March 2018, Pages 26-30
نویسندگان
Yu-Ri Park, Hye-Ryung Kim, Seong-Hee Kim, Kyoung-Ki Lee, Young S. Lyoo, Sang-Geon Yeo, Choi-Kyu Park,