کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8747159 | 1593426 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The detection of hepatitis c virus core antigen using afm chips with immobolized aptamers
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
In the present study, the possibility of hepatitis C virus core antigen (HCVcoreAg) detection in buffer solution, using atomic force microscope chip (AFM-chip) with immobilized aptamers, has been demonstrated. The target protein was detected in 1Â mL of solution at concentrations from 10â10Â Ð to 10â13Â Ð. The registration of aptamer/antigen complexes on the chip surface was carried out by atomic force microscopy (AFM). The further mass-spectrometric (MS) identification of AFM-registered objects on the chip surface allowed reliable identification of HCVcoreAg target protein in the complexes. Aptamers, which were designed for therapeutic purposes, have been shown to be effective in HCVcoreAg detection as probe molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 251, January 2018, Pages 99-105
Journal: Journal of Virological Methods - Volume 251, January 2018, Pages 99-105
نویسندگان
T.O. Pleshakova, A.L. Kaysheva, J.Ð. Bayzyanova, Ð.S. Anashkina, V.F. Uchaikin, V.S. Ziborov, V.A. Konev, A.I. Archakov, Y.D. Ivanov,