کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8749193 | 1593666 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Combination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients Combination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients](/preview/png/8749193.png)
چکیده انگلیسی
American Cutaneous leishmaniasis (ACL) is a public health problem. The immunological response is mainly dependent on T cell cytokine responses and might influence disease presentation, susceptibility and development. The understanding of the host immune response role in the control and in the pathology of leishmaniasis is relevant and has implications on diagnosis, follow-up and vaccine development. In this study, the differences in the immune response and T cell profile of patients before treatment was investigated through flow cytometry and real time PCR in peripheral blood mononuclear cells after different antigenic stimulations. Among the main findings are the significant presence of TNF and IFN-γ gene expression after 24â¯h of in vitro stimulation, and 48â¯h later the presence of CD4+ T and CD8+ T cells producing IL-10 and IL-4. This may be due to the differences in cytokine release over time and the presence of cells other than lymphocytes influencing the mRNA transcript detection. Evaluation of the immune response of individuals with leishmaniasis or other diseases should associate different technologies and times points for a clear and more reliable assessment of the immune response. This would help in the design of vaccine strategies/immunotherapies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 123, October 2018, Pages 433-439
Journal: Microbial Pathogenesis - Volume 123, October 2018, Pages 433-439
نویسندگان
Maria Carolina Accioly Brelaz-de-Castro, Thays Miranda de Almeida, Amanda Ferreira de Almeida, Marina de Assis Souza, Andresa Pereira de Oliveira Mendes, Rafael de Freitas e Silva, Maria Edileuza Felinto de Brito, Valéria Rêgo Alves Pereira,