کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8749423 | 1593669 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of Caco-2â¯cells response to Listeria monocytogenes virulence factors by RT-PCR
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Listeria monocytogenes expresses various virulence factors enabling the invasion and multiplying in host cells, and together induces cytokines transcription. In order to explore the relationship between virulence factors of L. monocytogenes wild-type EGD-e and cellular response in human colonic epithelial cell line(Caco-2), we constructed mutant strains with in-frame deletions of critical virulence genes of inlA, inlB, hly, actA and virulence regulatory factor prfA from EGD-e, respectively. Compared with EGD-e, mutant strains showed significantly decreased invasion and apoptosis in Caco-2â¯cells. However, mutant strains were capable to evoke cytokines transcription of interleukin-8 (IL-8), mononuclear chemoattractant protein-1 (MCP-1), tumor necrosis factor-a (TNF-a), interleukin-1β (IL-1β), interleukin-6 (IL-6) and CXCL-2 production in Caco-2â¯cells. Interestingly, EGD-e Îhly-infected Caco-2â¯cells showed a significant decrease of IL-6, IL-8 and MCP-1 transcription compared with EGD-e at 1â¯h post-infection. Simultaneously, EGD-e ÎinlB-infected cells showed a decrease in IL-6 transcription, while EGD-e ÎactA-infected cells reflected a decrease in MCP-1 transcription. Virulence genes play a role in inflammatory transcription, but the interaction between pathogenic bacteria and the host cells predominates in inflammatory transcription. Overall, the data showed cellular response of Caco-2â¯cells infected with EGD-e mutant strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 120, July 2018, Pages 79-84
Journal: Microbial Pathogenesis - Volume 120, July 2018, Pages 79-84
نویسندگان
Manman Xie, Chengchao Ding, Liang Guo, Guowei Chen, Haijuan Zeng, Qing Liu,