کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8750077 | 1593750 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pathogenicity locus determinants and toxinotyping of Clostridioides difficile isolates recovered from Iranian patients
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pathogenicity locus determinants and toxinotyping of Clostridioides difficile isolates recovered from Iranian patients Pathogenicity locus determinants and toxinotyping of Clostridioides difficile isolates recovered from Iranian patients](/preview/png/8750077.png)
چکیده انگلیسی
Little is known about the toxin profiles, toxinotypes and variations of toxin Clostridioides difficile C (tcdC) in Iranian C. difficile isolates. A total of 818 stool specimens were obtained from outpatients (n = 45) and hospitalized patients (n = 773) in Tehran, Iran, from 2011 to 2017. The 44 C. difficile isolates were subjected to PCR of toxin C. difficile A (tcdA), toxin C. difficile B (tcdB), tcdA 3â²-end deletion, toxinotyping and sequencing of the tcdC gene. Thirty-eight isolates (86.36%) were identified as tcdA and tcdB positive, and the remaining six isolates (13.63%) were nontoxigenic. All tcdA- and tcdB-positive isolates yielded an amplicon of 2535 bp by PCR for the tcdA 3â² end. Fourteen (36.84%), seventeen (44.73%) and seven (18.43%) isolates belonged to wild-type, toxin C. difficile C subclone3 (tcdC-sc3) and tcdC-A genotype of tcdC, respectively. Thirty-one isolates (81.57%) belonged to toxinotype 0, and seven isolates (18.42%) were classified as toxinotype V. This study provides evidence for the circulation of historical and hypervirulent isolates in the healthcare and community settings. Furthermore, it was also demonstrated that the tcdC-A genotype and toxinotype V are not uncommon among Iranian C. difficile isolates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Microbes and New Infections - Volume 25, September 2018, Pages 52-57
Journal: New Microbes and New Infections - Volume 25, September 2018, Pages 52-57
نویسندگان
A. Aliramezani, M. Talebi, A. Baghani, M. Hajabdolbaghi, M. Salehi, A. Abdollahi, S. Afhami, M. Marjani, F. Golbabaei, M.A. Boroumand, A. Sarrafnejad, M. Yaseri, S. Ghourchian, M. Douraghi,