کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8751506 | 1594200 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture](/preview/png/8751506.png)
چکیده انگلیسی
Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 517, April 2018, Pages 148-156
Journal: Virology - Volume 517, April 2018, Pages 148-156
نویسندگان
Adriaan H. de Wilde, Jessika C. Zevenhoven-Dobbe, Corrine Beugeling, Udayan Chatterji, Danielle de Jong, Philippe Gallay, Karoly Szuhai, Clara C. Posthuma, Eric J. Snijder,