کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8751790 | 1594307 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel virus (order Bunyavirales) from stressed redclaw crayfish (Cherax quadricarinatus) from farms in northern Australia
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Athtabvirus, a bunya-like virus and chequa iflavirus infect redclaw crayfish (Cherax quadricarinatus) and they may cause mortality reaching 20-40% after about three weeks following transportation stress. Lesions were seen in the muscles of broodstock and juveniles and nerve cords of craylings. Using NextGen sequencing, the whole transcriptomes of a farmed case crayfish and a tank-reared, unaffected crayfish were assembled producing over 500,000 contigs. The average depth of reads was 18 replicates with a range from 15 to 44. The near complete sequences of the large and middle genome segments of a bunya-like virus were detected along with chequa iflavirus. The internal bunya-like motifs; RNA-dependent RNA polymerase on the L segment, and glycoprotein n (Gn) on the M segment were easily identified. In the opposite, positive-sense direction on the M segment, another presumed glycoprotein (glycoprotein c) with a low-density lipoprotein receptor (cysteine-rich) motif was identified by position specific iterated (psi)-BLASTp. The athtabvirus was related to Whenzhou Shrimp Virus 2 (Eâ¯=â¯0.0, 43% amino acid identity), an unassigned, âve sense ssRNA virus, and to peribunyaviruses (Eâ¯=â¯10â50â20). In descending order of the number of RNA copies/0.2â¯mg of tissue, the organs most heavily infected were muscle (9.4â¯Ãâ¯106), nerve cord (5.24â¯Ãâ¯106), heart (4.07â¯Ãâ¯106), gills (3.96â¯Ãâ¯106), hepatopancreas (1.58â¯Ãâ¯106) and antennal gland (6.6â¯Ãâ¯105). Given the tissue tropism (muscle and nerves) of athtabvirus and the original lesions, this virus is implicated in being involved in the mortalities in crayfish after transportation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 250, 2 May 2018, Pages 7-12
Journal: Virus Research - Volume 250, 2 May 2018, Pages 7-12
نویسندگان
Kitikarn Sakuna, Jennifer Elliman, Anna Tzamouzaki, Leigh Owens,