کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8765573 | 1597722 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365-490Â nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photodiagnosis and Photodynamic Therapy - Volume 20, December 2017, Pages 201-206
Journal: Photodiagnosis and Photodynamic Therapy - Volume 20, December 2017, Pages 201-206
نویسندگان
Aziz ul Rehman, Ayad G. Anwer, Ewa M. Goldys,