In vivo visualization of gold-loaded cells in mice using x-ray computed tomography

The ability to perform cell tracking using x-ray computed tomography combined with gold nanoparticles has been demonstrated recently on ex vivo samples using different malignant and nonmalignant cell lines. Here we proved the concept of the method for in vivo assessment in a small-animal model of malignant brain tumors. The limitations of the method due to radiation dose constraints were investigated using Monte Carlo simulations. Taking into consideration different x-ray entrance doses and the spatial resolution, the visibility of the cell clusters was evaluated. The results of the experiments conducted on mice implanted with F98 tumor cells confirmed the prediction of the Monte Carlo calculations. Small clusters of cells exogenously loaded with gold nanoparticles could be visualized using our in vivo method.From the Clinical EditorThis article discusses the use of CT-based detection of gold nanoparticle loaded cells of interest in small-animal models of malignant brain tumors, where small clusters of cells loaded with gold nanoparticles could be visualized.

Graphical AbstractGold nanoparticles (GNPs) are conjugated with horse serum proteins to induce cellular uptake. F98 glioma cells are loaded with GNPs and then injected into mouse brains. One week after tumor cell implantation the tumor has grown and the concentration of the GNP marker has decreased. In vivo x-ray CT was used to measure the tumor volume and to follow its growth by measuring the x-ray attenuation.Figure optionsDownload high-quality image (136 K)Download as PowerPoint slide