کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8838773 | 1613215 | 2018 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitophagy is activated in brain damage induced by cerebral ischemia and reperfusion via the PINK1/Parkin/p62 signalling pathway
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کلمات کلیدی
ICAT1 weighted imagingT2 weighted imagingPTEN-induced kinase 1VDAC1T1WIT2WINVULC3DWIPINK1MCAONeuNECAADCGFAPi.p.CCAH&E - H & ECerebral ischemia-reperfusion injury - آسیب مجدد ایسکمی-ریپرفیز مغزیmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیDiffusion weighted imaging - تصویر برداری با وزن مخصوصintraperitoneal - داخل صفاقیBBB - سد خونی مغزیblood brain barrier - سد خونی مغزیexternal carotid artery - شریان کاروتید خارجیinternal carotid artery - شریان کاروتید داخلیcommon carotid artery - شریان کاروتید مشترکapparent diffusion coefficient - ضریب انتشار آشکارLAMP-1 - لامپ 1Mitophagy - میتوفاژیNeuronal nucleus - هسته عصبیhaematoxylin and eosin - هماتوکسیلین و ائوزینneurovascular unit - واحد عصبی عضلانیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالmicrotubule-associated protein 1 light chain 3 - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study examined the course of mitophagy following cerebral ischemia with reperfusion and the role of the PTEN-induced kinase 1 (PINK1)/Parkin/p62 signalling pathway. The middle cerebral artery of male Sprague-Dawley rats was occluded for 90â¯min and was followed by different time-points of reperfusion. Cerebral infarct areas were detected by 2,3,5-triphenyl tetrazolium chloride staining, while brain damage was observed by haematoxylin and eosin staining. Levels of LC3, Beclin1 and LAMP-1 were estimated by western blots. LC3B location was observed in various cells in the neurovascular unit. In addition, PINK1 accumulation in damaged mitochondria and Parkin/p62 mitochondrial translocation were investigated by double immunofluorescence staining. Finally, the levels of PINK1, Parkin and p62 expression in mitochondrial fractions were estimated by western blots. Cerebral ischemia with different time-points of reperfusion resulted in infarct in the territory of the middle cerebral artery accompanied by overall brain damage. In addition, we found up-regulation of LC3B, Beclin1, and LAMP-1, as well as mitophagy activation after reperfusion, with peak expression of these proteins at 24â¯h after reperfusion. Electron microscopy and immunofluorescence indicated that LC3B was primarily located in neurons, although lower levels of expression were found in astrocytes and even less in vascular endothelial cells. Moreover, significant increases in PINK1 accumulation in the outer membrane of mitochondria and increased Parkin/p62 mitochondrial translocation were shown at 24â¯h after reperfusion. These ï¬ndings suggest that the PINK1/Parkin/p62 signalling pathway was involved in the pathophysiological processes following ischemia and reperfusion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 142, September 2018, Pages 63-77
Journal: Brain Research Bulletin - Volume 142, September 2018, Pages 63-77
نویسندگان
Rui Lan, Ji-Tao Wu, Tao Wu, Yun-Zhi Ma, Bao-Qi Wang, Hai-Zhong Zheng, Ya-Na Li, Yan Wang, Chun-Qing Gu, Yong Zhang,