کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8839020 | 1613220 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analysis of lipid debris accumulation caused by cuprizone induced myelin degradation in different CNS areas
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کلمات کلیدی
Corpus callosumcuprizoneMOGEAEMAGdemyelination - demyelinimasexperimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیBoron-dipyrromethene - بور دیپیررومتینBODIPY - جسدCNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیgrey matter - ماده خاکستریCARS - ماشین هاCerebellum - مخچهGenome-wide association studies - مطالعات مرتبط با ژنومGWAS - مطالعهٔ همخوانی سراسر ژنومMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)coherent anti-Stokes Raman scattering - پراکندگی ضد استوکس رامان منسجمanterior commissure - کمربند قدامی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
Degradation of myelin sheath is thought to be the cause of neurodegenerative diseases, such as multiple sclerosis (MS), but definitive agreement on the mechanism of how myelin is lost is currently lacking. Autoimmune initiation of MS has been recently questioned by proposing that the immune response is a consequence of oligodendrocyte degeneration. To study the process of myelin breakdown, we induced demyelination with cuprizone and applied coherent anti-Stokes Raman scattering (CARS) microscopy, a non-destructive label-free method to image lipid structures in living tissue. We confirmed earlier results showing a brain region dependent myelin destructive effect of cuprizone. In addition, high resolution in situ CARS imaging revealed myelin debris forming lipid droplets alongwith myelinated axon fibers. Quantification of lipid debris with custom-made software for segmentation and three dimensional reconstruction revealed brain region dependent accumulation of lipid drops inversely correlated with the thickness of myelin sheaths. Finally, we confirmed that in situ CARS imaging is applicable to living human brain tissue in brain slices derived from a patient. Thus, CARS microscopy is potent tool for quantitative monitoring of myelin degradation in unprecedented spatiotemporal resolution during oligodendrocyte damage. We think that the accumulation of lipid drops around degrading myelin might be instrumental in triggering subsequent inflammatory processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 137, March 2018, Pages 277-284
Journal: Brain Research Bulletin - Volume 137, March 2018, Pages 277-284
نویسندگان
Attila Ozsvár, Róbert SzipÅcs, Zoltán Ozsvár, Judith Baka, Pál Barzó, Gábor Tamás, Gábor Molnár,