کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840701 | 1614694 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased Neuroligin 2 Levels in the Postsynaptic Membrane in Spinal Dorsal Horn may Contribute to Postoperative Pain
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کلمات کلیدی
cpsAMPAPWTPSD-95TTBSGluR1α-amino-3-hydroxy-5-methyl-4-isoxazole propionatesiRNA - siRNApaw withdrawal threshold - آستانه برداشتن پاsmall interfering ribonucleic acid - اسید ریبونوکلئیک دخالت کندPostoperative pain - درد پس از عملSpinal cord - طناب نخاعیpostsynaptic density protein 95 - پروتئین چگالی Postynaptic 95AMPA receptor - گیرنده AMPA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Increased Neuroligin 2 Levels in the Postsynaptic Membrane in Spinal Dorsal Horn may Contribute to Postoperative Pain Increased Neuroligin 2 Levels in the Postsynaptic Membrane in Spinal Dorsal Horn may Contribute to Postoperative Pain](/preview/png/8840701.png)
چکیده انگلیسی
Neuroligin 2 is a synaptic cell adhesion molecule that is mainly located in inhibitory synapses and is crucial in the regulation of synapse function through protein-protein interactions. However, researchers have not clearly determined whether neuroligin 2 is involved in the development of postoperative pain. In the current study, Western blot, immunofluorescence staining and co-immunoprecipitation were used to examine the critical role of neuroligin 2 in postoperative pain hypersensitivity. A small interfering ribonucleic acid (siRNA)-targeting neuroligin 2 was used to inhibit neuroligin 2 expression. Our data found that plantar incision induced postoperative pain hypersensitivity, which was characterized by paw withdrawal threshold and cumulative pain score. The upregulation of neuroligin 2 and GluR1 expression in the postsynaptic membranes of ipsilateral spinal dorsal horn was observed at 3â¯h and 1â¯day after plantar incision. Additionally, at 3â¯h after plantar incision, the amount of PSD-95 that was co-immunoprecipitated with neuroligin 2 antibody was significantly increased in the ipsilateral dorsal horn, as compared to that of the control group. Intrathecal pretreatment of siRNA-targeting neuroligin 2 to reduce the neuroligin 2 expression in the spinal cord significantly inhibited the pain hypersensitivity and reduced the synaptic targeting of GluR1 in ipsilateral dorsal horns. Our study indicates that the incision-induced interaction between neuroligin 2 and PSD-95 and subsequent synaptic targeting of GluR1 in ipsilateral dorsal horns contribute to postoperative pain hypersensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 382, 1 July 2018, Pages 14-22
Journal: Neuroscience - Volume 382, 1 July 2018, Pages 14-22
نویسندگان
Ruijuan Guo, Huili Li, Xueyang Li, Yuqing Sun, Huihui Miao, Danxu Ma, Fangxiao Hong, Ye Zhang, Yun Guan, Junfa Li, Ming Tian, Yun Wang,