کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840959 | 1614703 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two Weeks of Variable Stress Increases Gamma-H2AX Levels in the Mouse Bed Nucleus of the Stria Terminalis
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کلمات کلیدی
PBSgamma-H2AXγH2AXGSK3βBNSTDSBsMPFCMAPK - MAPKStress - استرس یا فشار روانیAnxiety - اضطرابdouble-strand breaks - شکست دو ردیفmedial prefrontal cortex - قشر غده پروسترولPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریbed nucleus of the stria terminalis - هسته تخت ترمینال های استریmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenglycogen synthase kinase 3 β - گلیکوزین سیتستاز کیناز 3 بتاGlycogen Synthase Kinase 3-beta - گلیکوژن سنتز کیناز 3 بتا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent reports demonstrate that DNA damage is induced, and rapidly repaired, in circuits activated by experience. Moreover, stress hormones are known to slow DNA repair, suggesting that prolonged stress may result in persistent DNA damage. Prolonged stress is known to negatively impact physical and mental health; however, DNA damage as a factor in stress pathology has only begun to be explored. Histone H2A-X phosphorylated at serine 139 (γH2AX) is a marker of DNA double-strand breaks (DSB), a type of damage that may lead to cell death if unrepaired. We hypothesized that a 14-day period of variable stress exposure sufficient to alter anxiety-like behavior in male C57BL/6J mice would produce an increase in γH2AX levels in the bed nucleus of the stria terminalis (BNST), a region implicated in anxiety and stress regulation. We observed that 14â¯days of variable stress, but not a single stress exposure, was associated with increased levels of γH2AX 24â¯h after termination of the stress paradigm. Further investigation found that phosphorylation levels of a pair of kinases associated with the DNA damage response, glycogen synthase kinase 3 β (GSK3β) and p38 mitogen-activated protein kinase (MAPK) were also elevated following variable stress. Our results suggest that unrepaired DNA DSBs and/or repetitive attempted repair may represent an important component of the allostatic load that stress places on the brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 373, 1 March 2018, Pages 137-144
Journal: Neuroscience - Volume 373, 1 March 2018, Pages 137-144
نویسندگان
Brendan D. Hare, Tina M. Thornton, Mercedes Rincon, Borivoj Golijanin, S. Bradley King, Diane M. Jaworski, William A. Falls,