کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841240 | 1614709 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Voluntary Binge-like Ethanol Consumption Site-specifically Increases c-Fos Immunoexpression in Male C57BL6/J Mice
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کلمات کلیدی
VTAvBNSTdBNSTVoluntary consumptionventral bed nucleus of the stria terminalisLPBNNTSPVAc-fosACBCPFABLAPBSPVTBECCRFNPYi.p.basolateral amygdala - amygdala basolateralCeA - CEACentral amygdala - آمیگدالای مرکزیEthanol - اتانولImmunoreactivity - ایمنی فعالintraperitoneal injection - تزریق داخل صفاقیtyrosine hydroxylase - تیروزین هیدروکسیلازDiD - دی دیHour - ساعتcorticotropin releasing factor - عامل آزاد کننده کورتیکوتروپینSurgery - عمل جراحیblood ethanol concentration - غلظت اتانول خونlocus coeruleus - لوکوس سیرولئوسPVN - مالیات بر ارزش افزودهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریventral tegmental area - ناحیه تگمنتوم شکمیbinge - نوشیدنیEdinger–Westphal nucleus - هستن Edinger-Westphalnucleus accumbens core - هسته accumbens هستهnucleus tractus solitarius - هسته دستگاه انفرادیlateral parabrachial nucleus - هسته پاراباژیال جانبی جانبیParaventricular nucleus of the thalamus - هسته پروانه مرغان تالاموسparaventricular nucleus of the hypothalamus - هسته پروانه مرکزی هیپوتالاموسlateral hypothalamus - هیپوتالاموس جانبیparaformaldehyde - پارافرمالدهیدNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The assessment of binge ethanol-induced neuronal activation, using c-Fos immunoreactivity (IR) as a marker of neuronal activity, is typically accomplished via forced ethanol exposure, such as intraperitoneal injection or gavage. Neuronal activity using a voluntary binge-like drinking model, such as “drinking-in-the-dark” (DID), has not been thoroughly explored. Additionally, studies assessing ethanol-elicited neuronal activation may or may not involve stereotaxic surgery, which could impact c-Fos IR. The experiments detailed herein aimed to assess the effects of voluntary binge-like ethanol consumption on c-Fos IR in brain regions implicated in ethanol intake in animals with and without surgery experience. Age-matched male C57BL/6J mice underwent either stereotaxic surgery (Study 1) or no surgery (Study 2). Then, mice experienced one 4-day DID cycle, tail blood samples were collected immediately after test conclusion on day 4, and mice were subsequently sacrificed. In each study, mice that drink ethanol were sorted into those that achieved binge-equivalent blood ethanol concentrations (BECsâ¯â¥â¯80â¯mg/dl) versus those that did not. Relative to water-consuming controls, mice with BECsâ¯â¥â¯80â¯mg/dl showed significantly elevated c-Fos IR in several brain regions implicated in neurobiological responses to ethanol. In general, the brain regions exhibiting binge-induced c-Fos IR were the same between studies, though differences were noted, highlighting the need for caution when interpreting ethanol-induced c-Fos IR when subjects have a prior history of surgery. Altogether, these results provide insight into the brain regions that modulate binge-like ethanol intake stemming from DID procedures among animals with and without surgery experience.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 367, 26 December 2017, Pages 159-168
Journal: Neuroscience - Volume 367, 26 December 2017, Pages 159-168
نویسندگان
Nathan W. Burnham, Todd E. Thiele,