کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8842003 | 1615037 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rab20, a novel Rab small GTPase that negatively regulates neurite outgrowth of PC12 cells
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کلمات کلیدی
mStreGFPNGFHRPPAGESmall GTPase - GTPase کوچکSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNApolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدearly endosome - اندوسوم اولیهMembrane traffic - ترافیک غشاءRab - ربNeurite outgrowth - رشد عصبیconstitutively active - سازنده فعال استPC12 cells - سلول های PC12endoplasmic reticulum - شبکه آندوپلاسمی nerve growth factor - فاکتور رشد عصبnot significant - مهم نیستKnockdown - نابودیlate endosome - پایان آندوسومHorseradish peroxidase - پراکسیداز هوررادیشenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Rab family small GTPases are key players in the membrane traffic that underlies various cellular phenomena. Neurite outgrowth, which is a prerequisite for neuronal network formation, also requires membrane traffic from the cell body to the tips of neurites. Although several Rabs have been shown to promote neurite outgrowth, very little is known about Rab involvement in the negative regulation of neurite outgrowth. Here we used nerve growth factor-stimulated PC12 cells to perform siRNA-based comprehensive knockdown screenings for Rabs that negatively regulate neurite outgrowth and succeeded in identifying Rab20 as a novel negative regulator of neurite outgrowth. Our findings showed that knockdown of endogenous Rab20 in PC12 cells promoted neurite outgrowth, whereas overexpression of active Rab20 inhibited it. We also found that the presence of Gly-64 and Cys-70, both of which are conserved only in the switch II region, a putative effector binding domain, of Rab20 is required for the inhibitory effect of Rab20 on neurite outgrowth. These findings indicated that active Rab20 suppresses neurite outgrowth of PC12 cells, possibly through interaction with an unidentified effector molecule that specifically recognizes certain amino acids in the switch II region of Rab20.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 662, 1 January 2018, Pages 324-330
Journal: Neuroscience Letters - Volume 662, 1 January 2018, Pages 324-330
نویسندگان
Mai E. Oguchi, Kan Etoh, Mitsunori Fukuda,