Enhanced removal of nitrate in the maize rhizosphere by plant growth-promoting Bacillus megaterium NCT-2, and its colonization pattern in response to nitrate

High soil nitrate concentrations can lead to the secondary salinization of soils. Bacillus megaterium NCT-2 is a wild-type strain isolated from secondary salinized soil and is very effective in reducing nitrate. Laboratory and greenhouse experiments were carried out to investigate its nitrate reduction capabilities, colonization pattern, and plant growth promotion responses to nitrate content in the soil. B. megaterium NCT-2 was marked with a green fluorescent protein (gfp) gene and was left to successfully colonize maize roots and the rhizosphere. Inoculation with gfp-tagged NCT-2 significantly promoted nitrate removal from the soil and improved plant growth. Confocal microscopy results revealed that NCT-2 is an endophyte that can colonize the meristematic and elongation zones of the root tip, and the middle segment of the root. Soil nitrate concentration had no significant effect on NCT-2 distribution. The gfp-tagged NCT-2 populations in the roots and rhizosphere soil first increased, but then decreased, and at the end of the experiment, colonization levels in the rhizosphere soil stabilized at ∼5 × 104 CFU g−1 soil. However, the levels in the roots increased again to 1-3 × 104 CFU g−1 root in the different treatments. The NCT-2 population in the roots was significantly affected by nitrate content. A nitrate-nitrogen concentration of 72 mg kg−1 was the optimum concentration for NCT-2 colonization of maize roots. This study will improve the agricultural application of NCT-2 as a biofertilizer for nitrate removal and plant growth promotion.