Selection of microalgae intended for valorization of digestate from agro-waste mixtures

Digestates have been recently recognized as valuable substrates for microalgal cultivation, effectively combining wastewater remediation and biofuels production. In this regard, selection of the appropriate species for such a process is of utmost importance. In this study, the performance of seven different microalgal strains in 10% (v/v) digestate which derived from the co-digestion of several agro-waste streams was investigated. Parachlorella kessleri, Acutodesmus obliquus, Chlorella vulgaris and Tetraselmis tetrathele were able to acclimate to this new medium, resulting in biomass yields and fatty acids (FAs) content which varied between 570-1117 mg L−1 and 3.9-24.5%, respectively. The main FAs detected in the four species were oleic, palmitic and linolenic acid, with significant differences in their relative abundance. Concerning nutrients removal, almost complete NH3-N removal was observed, while % TP removal exceeded 80% for three of the four strains tested. Furthermore, induction kinetics of prompt chlorophyll fluorescence was used as a screening tool indicative of the reactions of the photosynthetic machinery of different microalgal species cultivated in digestate.