کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8890186 | 1628502 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of UHPLC-HRMS methodology for the determination of flavonoids, amino acids and organosulfur compounds in black onion, a novel derived product from fresh shallot onions (Allium cepa var. aggregatum)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Black onion, a new derived product from fresh onion, has been developed by processing (aging) fresh shallot onion in a temperature- and humidity-controlled room without using any artificial additives. The aim of this study was to adapt, optimize and validate two ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) methodologies for the determination of flavonoids, amino acids and organosulfur compounds in black onion. UHPLC-HRMS methods involving RP-C18 and HILIC columns were adapted and validated in terms of specificity, linearity, limit of detection (LD) and quantification (LQ), precision inter- and intra-day, recovery and matrix effect. Linearity ranged from 0.012 to 12.5â¯ngμLâ1 and from 0.1 to 75â¯ngμLâ1 for flavonoid and amino acids and organosulfur compounds, respectively. LD varied from 0.004 to 0.06â¯ngμLâ1 and LQ from 0.012 to 0.2â¯ngμLâ1. The intra-day and inter-day precision for all compounds were less than 15% and the recovery ranged from 69 to 106%. The matrix effect ranged from 80 to 114% for flavonoids, amino acids and organosulfur compounds. The described methods were successfully applied for the correct separation and determination of 53 compounds in black onion. These results establish the value of these new two UHPLC-HRMS protocols in providing detailed compound profiles of black onion, highlighting their potential applicability to similar vegetables.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Volume 97, November 2018, Pages 376-383
Journal: LWT - Volume 97, November 2018, Pages 376-383
نویسندگان
José Manuel Moreno-Rojas, Alicia Moreno-Ortega, José Luis Ordóñez, Rafael Moreno-Rojas, Jesús Pérez-Aparicio, Gema Pereira-Caro,