کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8890856 | 1628505 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of growth tests employing a Îsod1 mutant of Saccharomyces cerevisiae to study the antioxidant activity of berry fruit extracts
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of the study was to evaluate the content of major bioactive compounds (BACs) and the antioxidant properties of aqueous berry extracts using ABTS
- + and DPPH
- biochemical assays and a novel bioassay based on the growth of a Îsod1 mutant of Saccharomyces cerevisiae on osmotic shock plates (OSPs). The extracts were prepared from raspberry ('Cascada Delight', 'Glen Fyne' and 'Octavia') cultivars, blackberry ('Navaho') cultivar, and wild blackberry, as well as a raspberry-blackberry hybrid. Concentrations of phenolic compounds, anthocyanins and ascorbic acid differed significantly in the fruit extracts, but were highest in the raspberry-blackberry hybrid. The antioxidant activity against DPPH
- and ABTS
- + among the berry cultivars and hybrid was highly dependent on the anthocyanin content. The Îsod1 yeast mutant growth test confirmed the antioxidant properties of all the extracts, with the variant based on counting of single colonies proving more sensitive than the spot test.
- + and DPPH
- biochemical assays and a novel bioassay based on the growth of a Îsod1 mutant of Saccharomyces cerevisiae on osmotic shock plates (OSPs). The extracts were prepared from raspberry ('Cascada Delight', 'Glen Fyne' and 'Octavia') cultivars, blackberry ('Navaho') cultivar, and wild blackberry, as well as a raspberry-blackberry hybrid. Concentrations of phenolic compounds, anthocyanins and ascorbic acid differed significantly in the fruit extracts, but were highest in the raspberry-blackberry hybrid. The antioxidant activity against DPPH
- and ABTS
- + among the berry cultivars and hybrid was highly dependent on the anthocyanin content. The Îsod1 yeast mutant growth test confirmed the antioxidant properties of all the extracts, with the variant based on counting of single colonies proving more sensitive than the spot test.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Volume 94, August 2018, Pages 96-102
Journal: LWT - Volume 94, August 2018, Pages 96-102
نویسندگان
Agata ÅwiÄciÅo, Kamila RybczyÅska-Tkaczyk, Agnieszka Najda, Anna KrzepiÅko, Roman Prażak, Grażyna ZawiÅlak,