کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8944984 | 1645266 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antimicrobial potential of Alpinia purpurata lectin (ApuL): Growth inhibitory action, synergistic effects in combination with antibiotics, and antibiofilm activity
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کلمات کلیدی
CFUSabouraud dextrose agarminimal fungicidal concentrationOrSAURMMBCMHAMICSDAMFCSDBMHb - MHBmethicillin-resistant S. aureus - S. aureus مقاوم به متیسیلینStaphylococcus aureus - استافیلوکوک اورئوسMRSA - استافیلوکوک اورئوس مقاوم به متی سیلین یا MRSA Oxacillin - اکساسیلینMinimal bactericidal concentration - حداقل غلظت ضد باکتریMinimal inhibitory concentration - حداقل غلظت مهار کنندهCeftazidime - سفتازیدیمPseudomonas aeruginosa - سودوموناس آئروژینوزاHemagglutinating activity - فعالیت hemagglutinatingFluconazole - فلوکونازولSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیMueller Hinton Agar - مولر هینتون آگارMueller Hinton Broth - مولر هینتون براثScanning electron microscopy - میکروسکوپ الکترونی روبشیcolony-forming units - واحدهای تشکیل دهنده کلنیPropidium iodide - پروتئین یدیدoptical density - چگالی نوریCandida - کاندیداPositive control - کنترل مثبتnegative control - کنترل منفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Alpinia purpurata inflorescence contains a lectin (ApuL), which has immunomodulatory activities on human cells. In the present work, it was evaluated the antibacterial and antifungal effects of ApuL against human pathogens. ApuL showed bacteriostatic activity against non-resistant (UFPEDA-02) and an oxacillin-resistant isolate (UFPEDA-672) of Staphylococcus aureus with minimal inhibitory concentrations (MIC50) of 50 and 400â¯Î¼g/mL, respectively. In addition, it showed bactericidal effect on the non-resistant isolate (minimal bactericidal concentration: 200â¯Î¼g/mL). For Candida albicans and Candida parapsilosis, ApuL showed fungistatic effect (MIC50: 200 and 400â¯Î¼g/mL, respectively). The lectin was able to impair the viability of the microorganism cells, as indicated by propidium iodide (PI) staining. Analysis of growth curves, protein leakage, and ultrastructural changes supported that ApuL acts through distinct mechanisms on S. aureus isolates. Ultrastructural analysis of ApuL-treated Candida cells revealed malformations with elongations and bulges. ApuL-oxacillin combination showed synergistic effect on the oxacillin-resistant isolates UFPEDA-670 and 671, which were not sensitive to lectin alone. Synergism was also detected for ApuL-ceftazidime against a multidrug-resistant isolate of Pseudomonas aeruginosa. Synergistic action of ApuL-fluconazole was detected for C. parapsilosis, which was insensitive to the drug alone. Biofilm formation by S. aureus non-resistant isolate and C. albicans was remarkably inhibited by ApuL at sub-inhibitory concentrations. In conclusion, ApuL showed differential effects on non-resistant and resistant bacterial isolates, was active against Candida species, and showed synergistic action in combination with antibiotics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 124, November 2018, Pages 152-162
Journal: Microbial Pathogenesis - Volume 124, November 2018, Pages 152-162
نویسندگان
Gustavo Ramos Salles Ferreira, Jéssica de Santana Brito, Thamara Figueiredo Procópio, Nataly Diniz de Lima Santos, Bárbara José Rocha Cardoso de Lima, Luana Cassandra Breitenbach Barroso Coelho, Daniela Maria do Amaral Ferraz Navarro,