کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8947789 1645610 2018 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A dual-channel homogeneous aptasensor combining colorimetric with electrochemical strategy for thrombin
ترجمه فارسی عنوان
کانون یکنواخت کانال دو کاناله با ترکیب رنگ سنجی با استراتژی الکتروشیمیایی برای ترومبین
کلمات کلیدی
کانال دوگانه آپتا سنسور همگن، الکتروشیمیایی، رنگ سنجی، نانوذرات هیدروکسی آپاتیت،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in a single analytical system for signal readout. In this dual-channel sensing strategy, the G-quadruplex sequence was released and incorporated with hemin to form DNAzyme for naked-eye colorimetric detection. Meanwhile, the hydroxyapatite nanoparticle as signal probe was combined with magnetic nanoparticles to construct sandwich-type structure for generating the electrochemical current when thrombin was present in solution. By introducing two kinds of reporter probes and transducers, this dual-channel sensor produced two different kinds of signal to improve the analytical accuracy and diversity. The results revealed that the dual-channel sensor achieved the quantatitive determination of thrombin with low limit of detection (0.40 fM) and wide range (0.1 fM to 1 nM), which offer a promise for rapid and accurate detection of biomolecule.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 120, 30 November 2018, Pages 15-21
نویسندگان
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