کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8947789 | 1645610 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dual-channel homogeneous aptasensor combining colorimetric with electrochemical strategy for thrombin
ترجمه فارسی عنوان
کانون یکنواخت کانال دو کاناله با ترکیب رنگ سنجی با استراتژی الکتروشیمیایی برای ترومبین
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کلمات کلیدی
کانال دوگانه آپتا سنسور همگن، الکتروشیمیایی، رنگ سنجی، نانوذرات هیدروکسی آپاتیت،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in a single analytical system for signal readout. In this dual-channel sensing strategy, the G-quadruplex sequence was released and incorporated with hemin to form DNAzyme for naked-eye colorimetric detection. Meanwhile, the hydroxyapatite nanoparticle as signal probe was combined with magnetic nanoparticles to construct sandwich-type structure for generating the electrochemical current when thrombin was present in solution. By introducing two kinds of reporter probes and transducers, this dual-channel sensor produced two different kinds of signal to improve the analytical accuracy and diversity. The results revealed that the dual-channel sensor achieved the quantatitive determination of thrombin with low limit of detection (0.40â¯fM) and wide range (0.1â¯fM to 1â¯nM), which offer a promise for rapid and accurate detection of biomolecule.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 120, 30 November 2018, Pages 15-21
Journal: Biosensors and Bioelectronics - Volume 120, 30 November 2018, Pages 15-21
نویسندگان
Yaxing Zhang, Jianfei Xia, Feifei Zhang, Zonghua Wang, Qingyun Liu,