کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8949250 1645702 2018 41 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Plastoquinol generates and scavenges reactive oxygen species in organic solvent: Potential relevance for thylakoids
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Plastoquinol generates and scavenges reactive oxygen species in organic solvent: Potential relevance for thylakoids
چکیده انگلیسی
The present work reports reactions of plastoquinol (PQH2-9) and plastoquinone (PQ-9) in organic solvents and summarizes the literature to understand similar reactions in thylakoids. In thylakoids, PQH2-9 is oxidized by the cytochrome b6/f complex (Cyt b6/f) but some PQH2-9 is also oxidized by reactions in which oxygen acts as an electron acceptor and is converted to reactive oxygen species (ROS). Furthermore, PQH2-9 reacts with ROS. Light enhances oxygen-dependent oxidation of PQH2-9. We examined the oxidation of PQH2-9 via dismutation of PQH2-9 and PQ-9 and scavenging of the superoxide anion radical (O2−) and hydrogen peroxide (H2O2) by PQH2-9. Oxidation of PQH2-9 via dismutation to semiquinone was slow and independent of pH in organic solvents and in solvent/buffer systems, suggesting that intramembraneous oxidation of PQH2-9 in darkness mainly proceeds via reactions catalyzed by the plastid terminal oxidase and cytochrome b559. In the light, oxidation of PQH2-9 by singlet oxygen and by O2− formed in PSI contribute significantly. In addition, Cyt b6/f forms H2O2 with a PQH2-9 dependent mechanism. Measurements of the reaction of O2− with PQH2-9 and PQ-9 in acetonitrile showed that O2− oxidizes PQH2-9, forming PQ-9 and several PQ-9-derived products. The rate constant of the reaction between PQH2-9 and O2− was found to be 104 M−1 s−1. H2O2 was found to oxidize PQH2-9 to PQ-9, but failed to oxidize all PQH2-9, suggesting that the oxidation of PQH2-9 by H2O2 proceeds via deprotonation mechanisms producing PQH−-9, PQ2−-9 and the protonated hydrogen peroxide cation, H3O2+.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 10, October 2018, Pages 1119-1131
نویسندگان
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