کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8949257 | 1645702 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PROTON GRADIENT REGULATION 5 contributes to ferredoxin-dependent cyclic phosphorylation in ruptured chloroplasts
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کلمات کلیدی
PGR5DCMUNDHantimycin ADTTCEFPMFPPFDFCCP3-(3,4-Dichlorophenyl)-1,1-dimethylurea - 3- (3،4-Dichlorophenyl) -1،1-dimethylureaECs - EC هاElectrochromic shift - تغییر الکتروکرومیLinear electron flow - جریان الکتریکی خطیCyclic electron flow - جریان الکتریکی سیسیکdithiothreitol - دیتیوتریتولATP synthesis - سنتز ATPcyt - سیتcytochrome - سیتوکرومPhotosynthesis - فتوسنتزphotosynthetic photon flux density - فتوسنتز تراکم شار فوتونFerredoxin - فریدوکسیینLef - لفMethyl viologen - متیل ویولونگproton motive force - نیروی حرکتی پروتونMembrane potential - پتانسیل غشاء plastoquinone - پلواستیکینونcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazone
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Antimycin A-sensitive cyclic electron flow (CEF) was discovered as cyclic phosphorylation by Arnon et al. (1954). Because of its sensitivity to antimycin A, PROTON GRADIENT REGULATION 5 (PGR5)/PGR5-like Photosynthetic Phenotype 1 (PGRL1)-dependent CEF has been considered identical to the CEF of Arnon et al. However, this conclusion still needs additional supportive evidence, mainly because of the absence of definitive methods of evaluating CEF activity. In this study, we revisited the classical method of monitoring cyclic phosphorylation in ruptured chloroplasts to characterize two Arabidopsis mutants: pgr5, which is defective in antimycin A-sensitive CEF, and chlororespiratory reduction 2-1 (crr2-1), which is defective in chloroplast NDH-dependent CEF. We observed a significant reduction in CEF-dependent pmf formation and consequently ATP synthesis in the pgr5 mutant, although LEF-dependent pmf formation and ATP synthesis were not impaired at photosynthetic photon flux densities below 130â¯Î¼molâ¯mâ2â¯sâ1. In contrast, the contribution of chloroplast NDH complex to pmf formation and ATP synthesis was not significant. Antimycin A partially inhibited CEF-dependent pmf formation, although there may be further inhibition sites. Unlike in the observation in leaves, the proton conductivity of ATP synthase, monitored as gH+, was not enhanced in ruptured chloroplasts of the pgr5 mutant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 10, October 2018, Pages 1173-1179
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 10, October 2018, Pages 1173-1179
نویسندگان
Caijuan Wang, Hiroko Takahashi, Toshiharu Shikanai,