کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8974587 1552992 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface disinfection of cod Gadus morhua and haddock Melanogrammus aeglefinus eggs with bronopol
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Surface disinfection of cod Gadus morhua and haddock Melanogrammus aeglefinus eggs with bronopol
چکیده انگلیسی
Using current practice of immersion, the survival of cod eggs disinfected with 50 mg l− 1 to 500 mg l− 1 bronopol (as Pyceze [50%W/V bronopol], Novartis Animal Vaccines Ltd.) for 45 s, was higher (83-89%) when compared with negative controls (48%). Hatching larvae survived exposure to Pyceze but not to Kickstart (25% peracetic acid/hydrogen peroxide, CIDLines UK), a common disinfectant in United Kingdom marine finfish hatcheries. Bacterial numbers on cod eggs were 30 CFU ml− 1 following contact with 50 mg l− 1 bronopol and zero at 1000 mg l− 1 compared with 14,000 CFU ml− 1 with no disinfection. Although survival to hatch of haddock eggs in 50 mg− 1 bronopol (71.6%) was higher than in non-disinfected eggs (62.4%) this was not significant. Haddock eggs required a concentration of 500 mg l− 1 bronopol to achieve a significant reduction in bacterial numbers, 352 cf. 1546 CFU ml− 1 in seawater controls. Alternative techniques to immersion were assessed to reduce repeated handling and possible damage to eggs. A flow-through system utilising a peristaltic dosing pump was ineffective in achieving the target concentration of Pyceze and contact time, but use of a static immersion technique in an incubation unit, with 30 min daily exposure to 50 mg l− 1 bronopol, gave 63% survival to hatch compared with 35% with a negative control. Bacterial numbers of 892 CFU ml− 1 following treatment with Pyceze were significantly lower than the 91,400 CFU ml− 1 in negative controls. In target animal safety studies with 250 mg l− 1 bronopol, namely × 5 target concentration for 2× exposure time, survival of haddock eggs of 88.7% was significantly higher when compared with 53.1% in non-disinfected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 250, Issues 1–2, 14 November 2005, Pages 27-35
نویسندگان
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