کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8979170 | 1107110 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PCR detection of cows' milk in water buffalo milk and mozzarella cheese
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Polymerase chain reaction (PCR) has been applied for the specific detection of cows' DNA in water buffalo milk and mozzarella cheese by using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of specific primers for cow yielded a 346Â bp fragment from cows' milk DNA, whereas no amplification signal was obtained in sheep's, goats' and water buffalo's milk DNA. Analysis of both buffalo milk and buffalo mozzarella cheese mixtures containing different percentages of cows' milk or bovine mozzarella cheese, enabled the specific detection of cow's DNA with a sensitivity threshold of 0.1%. The proposed PCR assay represents a rapid and straightforward method for the detection of adulterations in water buffalo milk and mozzarella cheese.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Dairy Journal - Volume 15, Issue 11, November 2005, Pages 1122-1129
Journal: International Dairy Journal - Volume 15, Issue 11, November 2005, Pages 1122-1129
نویسندگان
I. López-Calleja, I. González Alonso, V. Fajardo, M.A. RodrÃguez, P.E. Hernández, T. GarcÃa, R. MartÃn,