کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8981302 | 1108156 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Kinetics of Heat-Induced Structural Changes of β-Lactoglobulin
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The Kinetics of Heat-Induced Structural Changes of β-Lactoglobulin The Kinetics of Heat-Induced Structural Changes of β-Lactoglobulin](/preview/png/8981302.png)
چکیده انگلیسی
Heat-induced structural changes of β-lactoglobulin were studied at temperatures ranging from 67.5 to 82.5°C, and at pH 7.5. These changes were monitored by measurement of surface hydrophobicity, thiol availability, and protein solubility. Kinetic studies were conducted to quantitatively describe the contribution of hydrophobic and SH/SS interchange reactions to the thermal structural changes of β-lactoglobulin. Results indicate that β-lactoglobulin is sensitive to heat-induced interchange reactions with consequences for protein solubility. The extent of changes measured by the increase in surface hydrophobicity and the decrease in slow-reacting SH groups content could be described by a first-order fractional conversion model and were characterized by activation energy values of 233.9 ± 8.6 and 148.2 ± 6.7 kJ/mol, respectively. The break in the Arrhenius plot suggested in literature for β-lactoglobulin denaturation was confirmed in this study only for the kinetics of exposed SH groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 88, Issue 5, May 2005, Pages 1646-1653
Journal: Journal of Dairy Science - Volume 88, Issue 5, May 2005, Pages 1646-1653
نویسندگان
N. Sava, I. Van der Plancken, W. Claeys, M. Hendrickx,