کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8983703 1109722 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of raw meats and fats of pigs using polymerase chain reaction for Halal authentication
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Analysis of raw meats and fats of pigs using polymerase chain reaction for Halal authentication
چکیده انگلیسی
A method for species identification from pork and lard samples using polymerase chain reaction (PCR) analysis of a conserved region in the mitochondrial (mt) cytochrome b (cyt b) gene has been developed. Genomic DNA of pork and lard were extracted using Qiagen DNeasy® Tissue Kits and subjected to PCR amplification targeting the mt cyt b gene. The genomic DNA from lard was found to be of good quality and produced clear PCR products on the amplification of the mt cyt b gene of approximately 360 base pairs. To distinguish between species, the amplified PCR products were cut with restriction enzyme BsaJI resulting in porcine-specific restriction fragment length polymorphisms (RFLP). The cyt b PCR-RFLP species identification assay yielded excellent results for identification of pig species. It is a potentially reliable technique for detection of pig meat and fat from other animals for Halal authentication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 69, Issue 1, January 2005, Pages 47-52
نویسندگان
, , , , ,