کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8991015 1555805 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-dimensional electrophoresis and mass spectrometry for the identification of species-specific Trichinella antigens
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Two-dimensional electrophoresis and mass spectrometry for the identification of species-specific Trichinella antigens
چکیده انگلیسی
A proteomic approach was utilized for fine antigenic characterization of the closely related Trichinella genotypes Trichinella britovi T3 and Trichinella T8. Crude extract of muscle larvae L1 (LCE) from both isolates were analyzed by 2D-PAGE. Over 500 protein spots were reproducibly separated in both genotypes. These separated proteins were identified in Western blot with IgG1 and IgG3 from homologous and heterologous hyperimmune sera raised in BALB/c mice. A group of 20 and 15 spots migrating at 50-60 kDa and pH 5.5-6.5 in T. britovi and Trichinella T8 maps, respectively, reacted with the IgG1 from heterologous sera whereas a group of minor spots of similar migration patterns did not. Low cross-reactivity occurred for IgG3. MALDI-TOF and MALDI-TOF/TOF analysis of these antigens identified a predicted enolase, the protein P49 and a predicted actin among the cross-reactive proteins and two hypothetical actins among the non cross-reactive proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 132, Issues 1–2, 5 September 2005, Pages 43-49
نویسندگان
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