کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8994470 1114416 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of PPAR, RXR Isoforms and Fatty Acid Transporting Proteins in the Rat and Human Gastrointestinal Tracts
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Expression of PPAR, RXR Isoforms and Fatty Acid Transporting Proteins in the Rat and Human Gastrointestinal Tracts
چکیده انگلیسی
Dietary fatty acid (FA) absorption across the gastrointestinal (GI) tract is of critical importance for sustenance, however, excessive FA absorption has also been linked to metabolic syndrome and associated disorders. The expression of isoforms that regulate the dietary FA absorption are not as well characterized in the GI tract as they are elsewhere. Peroxisome proliferator-activated receptors (PPARα, β, and γ) and 9-cis-retinoic acid receptors (RXRα, β, and γ) are nuclear hormone transcription factors that control FA homeostasis, in part through the regulation of expression of membrane-bound FA transporting proteins. The present study was designed to elucidate the expression of PPAR and RXR isoforms and FA transporting proteins (FABPpm and FAT/CD36) in the rat and human GI tracts using reverse transcriptase-polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemical staining. The results revealed rat GI expression of all the PPAR and RXR isoforms, FABPpm and FAT/CD36. PPARα, PPARβ, PPARγ, RXRα, FABPpm, and FAT/CD36 isoforms exhibited ubiquitous expression in human GI tract, whereas RXRβ was not detected. RXRγ was observed in a majority of the human GI samples. These results provide a physiological foundation for rational drug design and drug delivery for the mitigation of metabolic syndrome and associated disorders to normalize intestinal FA absorption.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 94, Issue 2, February 2005, Pages 363-372
نویسندگان
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