کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9001428 1118516 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CYP2C-catalyzed delta(9)-tetrahydrocannabinol metabolism: Kinetics, pharmacogenetics and interaction with phenytoin
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
CYP2C-catalyzed delta(9)-tetrahydrocannabinol metabolism: Kinetics, pharmacogenetics and interaction with phenytoin
چکیده انگلیسی
delta(9)-Tetrahydrocannabinol (Δ9-THC), the primary psychoactive constituent of marijuana, is subject to first pass hepatic metabolism primarily by hydroxylation to yield active and inactive oxygenated products. The primary metabolite is formed via oxidation of the allylic methyl group to yield 11-hydroxy-Δ9-THC, which is oxidized further to 11-nor-9-carboxy-Δ9-THC. The hydroxylation is thought to be mediated by CYP2C9. The present study was designed to address the kinetics and pharmacogenetics of CYP2C-mediated metabolism of (Δ9)-THC by studying its metabolism in human liver microsomes and expressed enzymes. Expressed CYP2C9.1 exhibited high affinity for the hydroxylation of Δ9-THC (apparent Km, 2 μM), similar to that observed in human liver microsomes (apparent Km, 0.8 μM). In contrast, the calculated intrinsic clearance (apparent Vm/Km) for CYP2C9.2 and CYP2C9.3 was approximately 30% that of the wild type, CYP2C9.1. Given the high affinity of CYP2C9 for the hydroxylation of Δ9-THC, we evaluated the potential for an interaction between Δ9-THC, 11-hydroxy-Δ9-THC, or 11-nor-9-carboxy-Δ9-THC and the CYP2C9 substrate, phenytoin. Surprisingly, Δ9-THC increased the rate of phenytoin hydroxylation in human liver microsomes and expressed CYP2C9 enzyme. Similar increases in rate were observed with co-incubation of 11-hydroxy-Δ9-THC and 11-nor-9-carboxy-Δ9-THC with phenytoin. These in vitro data suggest the potential for an interaction from the concomitant administration of Δ9-THC and phenytoin that could result in decreased phenytoin concentrations in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 70, Issue 7, 1 October 2005, Pages 1096-1103
نویسندگان
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