کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9001905 | 1118561 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Non-stimulated Ca2+ leak pathway in cerebellar granule neurones
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to investigate the pathways of calcium influx routes in non-stimulated cerebellar granule neurones by use of standard microspectrofluorimetric techniques. Repetitive application of Ca2+-free solutions for various time intervals induced decreases of resting cytosolic free Ca2+ concentration ([Ca2+]i) which were followed, on Ca2+ readmission, by a full recovery, always to the initial resting [Ca2+]i levels. Use of drugs to deplete calcium stores (thapsigargin, alone or combined with low levels of ionomycin) did not cause release of Ca2+ from the intracellular stores nor enhanced the activity of the Ca2+ entry pathway. This influx was mainly independent of voltage operated calcium channels, since both L-type channel blockers (nitrendipine) and the hyperpolarizing agent pinacidil (a K+-channel opener) were without effect. Contribution from glutamate receptors to this influx was eliminated since a combination of blockers of NMDA and AMPA glutamate receptors (NBQX and D-AP5) did not affect the properties of the Ca2+ response. The Ca2+ leak pathway was sensitive to micromolar levels of lanthanum and gadolinium, and to the compound 2-APB, features shared by several channels of the TRP superfamily. In summary, our results show the presence of a Ca2+ permeable pathway, active and patent in resting conditions in cerebellar granule neurones, and which is different from the voltage-operated calcium channels and not operated by depletion of the stores.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 70, Issue 5, 1 September 2005, Pages 786-793
Journal: Biochemical Pharmacology - Volume 70, Issue 5, 1 September 2005, Pages 786-793
نویسندگان
P.J. Gómez Pinilla, A.T. Hernández, M.C. Camello, M.J. Pozo, E.C. Toescu, P.J. Camello,