کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9007840 | 1559768 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of histamine H1 and H2 receptors in the regulation of STAT-1 phosphorylation: Inverse agonism exhibited by the receptor antagonists
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کلمات کلیدی
ICAM-1pKaH89PKCdb-cAMPcAMP - cAMPPMA - LDC هاStat1 - sTAT1Cyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicAsthma - آسمInverse agonism - آگونیسم معکوسsignal transducer and activator of transcription-1 - فرستنده سیگنال و فعال کننده رونویسی-1intracellular adhesion molecule-1 - مولکول چسبندگی داخل سلولی -1Crosstalk - هم شنوی، کراس تاک، نشت سیگنالHistamine - هیستامینprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Involvement of histamine H1 and H2 receptors in the regulation of STAT-1 phosphorylation: Inverse agonism exhibited by the receptor antagonists Involvement of histamine H1 and H2 receptors in the regulation of STAT-1 phosphorylation: Inverse agonism exhibited by the receptor antagonists](/preview/png/9007840.png)
چکیده انگلیسی
Signal transducer and activator of transcription-1 (STAT1) is a latent signal transducer protein which, on phosphorylation, is translocated from the cytoplasm to the nucleus and is subsequently activated. This study was designed to determine the involvement of histamine receptors in histamine-mediated effect on STAT1 phosphorylation. It is known that the actions of histamine mediated through H1 and H2 receptors are dependent on their respective downstream pathways, Ca2+-PKC and cAMP-PKA. In this study, we investigated the significance of PKA in STAT1 phosphorylation. C57BL/6 mouse splenocytes were isolated and treated with histamine (10â 7-10â 4 M) and then activated with PMA (phorbol 12 myristate 13-acetate) plus ionomycin. The phosphorylated STAT1 levels were analyzed by immunoblotting. Histamine receptor agonists amthamine and betahistine, histamine receptor antagonists pyrilamine maleate, tripelennamine, ranitidine, cimetidine and thioperamide, cAMP agonists N6, 2â²-0-dibutyryladenosine-3â²,5â²-cyclic monophosphate sodium salt (db-cAMP) and forskolin, protein kinase A inhibitors N-(2-[p-bromocinnamylamino]ethyl)-5-isoquinoline-sulfonamide (H89) and Rp diastereomer of adenosine cyclic 3â²,5â²-phosphorothioate (RpcAMPs) and tyrosine kinase inhibitor tyrphostin were used to identify the upstream signal transduction pathways. We observed that histamine augmented the phosphorylation of STAT1 through both H1 and H2 receptors. Furthermore, H1 and H2 receptor antagonists displayed inverse agonism. Ca2+-PKC-induced phosphorylation of STAT1 was completely inhibited by H89 and significantly inhibited by RpcAMPs. DbcAMP and forskolin augmented the Ca2+-PKC-induced STAT1 phosphorylation thus suggesting a convergent crosstalk between the two histamine receptor signaling pathways, PKA and PKC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 5, Issues 7â8, July 2005, Pages 1299-1309
Journal: International Immunopharmacology - Volume 5, Issues 7â8, July 2005, Pages 1299-1309
نویسندگان
Shilpa P. Sakhalkar, Eric B. Patterson, Manzoor M. Khan,