کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9015176 | 1127138 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of 4-aminopyridine (4-AP) on the spontaneous activity and neuromuscular junction in the rat colon
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of 4-aminopyridine (4-AP) on the spontaneous activity and neuromuscular junction in the rat colon Effect of 4-aminopyridine (4-AP) on the spontaneous activity and neuromuscular junction in the rat colon](/preview/png/9015176.png)
چکیده انگلیسی
Two pacemakers are involved in the generation of the spontaneous electrical activity in the rat colon. Slow waves, originated near the submuscular plexus, elicit high frequency contractions whereas low frequency contractions are related to cyclic depolarizations, which might be originated near the Auerbach's plexus. Inhibitory junction potentials (IJPs) in the rat colon show a fast component followed by a sustained NO-mediated hyperpolarization. We have utilised mechanical recordings and intracellular electrophysiology to characterize the effects of 4-AP on spontaneous electrical activity and inhibitory neurotransmission in isolated preparations of rat colon. 4-AP reduced the repolarization of smooth muscle cells and caused a transient (for 4-5 min) depolarization (9.7 ± 3.1 mV; n = 4) and repetitive spikes. The mechanical activity correlated with these electrical changes consisted of a transient increase in tone without cyclic activity followed by long lasting high amplitude cyclic contractions. To avoid smooth muscle cyclic depolarizations, nifedipine 1 μM was added to the perfusion solution. 4-AP 5 mM hyperpolarized (â11.4 ± 2.1 mV, n = 5) the smooth muscle and induced spontaneous IJPs. This effect was not observed when the tissue was preincubated with TTX 1 μM (n = 7) and L-NNA 1 mM (n = 4). We conclude that 4-AP inhibits repolarization of smooth muscle cells and induces release of NO from nerve endings. This effect might be due to inhibition of K+ channels both in neurons and smooth muscle cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacological Research - Volume 52, Issue 6, December 2005, Pages 447-456
Journal: Pharmacological Research - Volume 52, Issue 6, December 2005, Pages 447-456
نویسندگان
E. AlbertÃ, M. Jiménez,