کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9017684 | 1128659 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lead enhances CD4+ T cell proliferation indirectly by targeting antigen presenting cells and modulating antigen-specific interactions
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کلمات کلیدی
TCrAPCBLLMIICMLCalloantigen - آلوآنتیژنAntigen presenting cells - آنتیژن ارائه سلولInterleukin-2 - اینترلوکین-۲Lead - سربBlood lead level - سطح سرب خونantigen presenting cell - سلولهای پردازنده آنتیژنImmunotoxicity - سمیت ایمنیMixed lymphocyte culture - فرهنگ لنفوسیت مختلطMHC - مجموعه سازگاری بافتی اصلیmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیTransgenic mice - موش ترانس ژنیکMajor histocompatibility complex class II - کلاس پیچیده بافتی عمده IIClip - کلیپT cell receptor - گیرنده سلول T
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Although Pb is a well-known immunotoxicant, its mechanism of action is not well understood. Low levels of Pb (â¼1 μM) markedly enhance the proliferative T cell response in mixed lymphocyte culture (MLC), a process we have termed allo-enhancement. As Pb allo-enhancement occurs whether alloantigen presenting cells (APC) are derived from C57BL/6 or BALB.B10, the allo-reactive T cells involved are likely to be specific for peptide in the context of the IAb molecule as the IE molecule is null in H-2b mice. Analysis of T cell division in MLC with Pb treatment indicated that there was no significant difference between Pb and non-Pb-treated cultures until day 4 when the frequency of proliferating T cells was much greater than in non-treated cultures. Our data suggest that this increased proliferation is not coupled with increased IL-2 levels in the media as these were actually decreased with Pb treatment and that Pb-induced enhancement in the allo-proliferative response is only partially dependent upon IL-2. Pb allo-enhancement is abrogated when stimulating allo-APCs are paraformaldehyde-fixed, and T cell proliferation stimulated by concanavalin A is not enhanced with Pb treatment, suggesting that the APC is the proximate target of Pb in allo-MLC. Pb allo-enhancement does not occur when T cells respond to irradiated allo-B cells, alone; however, it is restored when syngeneic CD11c-enriched cells are added. Of the CD11c-enriched splenocytes, the fraction that is adherent after 24 h, consistent with macrophages, appears to be the cell type targeted by Pb. Using T cells from DO11.10 transgenic mice, we determined that the effect of Pb is centered around specific p:MHC interactions and that enhanced costimulation is an unlikely mechanism for Pb allo-enhancement.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 2, 1 September 2005, Pages 125-137
Journal: Toxicology and Applied Pharmacology - Volume 207, Issue 2, 1 September 2005, Pages 125-137
نویسندگان
David G. Farrer, Sara M. Hueber, Michael J. Jr.,