کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9030713 | 1130940 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Safety and efficacy evaluation of aqueous citric acid to degrade B-aflatoxins in maize
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کلمات کلیدی
AFB2AL2AFB1 - AFB 1DMSO - DMSOAflatoxin B1 - آفلاتوکسین B1Aflatoxin B2 - آفلاتوکسین B2analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceAmes test - تست ایمز Acidic treatment - درمان اسیدیdimethyl-sulfoxide - دی متیل سولفوکسیدRetention time - زمان بازداریDetoxification - سم زداییMoisture content - محتوای رطوبتhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chemical inactivation of aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) in maize grain by means of 1 N aqueous citric acid was confirmed by the AFLATEST⢠immunoaffinity column method, high performance liquid chromatography (HPLC), and the Ames test (Salmonella-microsomal screening system). The AFLATEST⢠assay showed that aflatoxins in the maize grain with an initial concentration of 29 ng/g were completely degraded and 96.7% degradation occurred in maize contaminated with 93 ng/g when treated with the aqueous citric acid. Aflatoxin fluorescence strength of acidified samples was much weaker than untreated samples as observed in HPLC chromatograms. On the other hand, the Ames test results indicated that the mutagenic activity of acidified samples was greatly reduced compared with that of untreated samples based on hisâ â his+ reversions in the Salmonella TA100 strain. Chemical inactivation appears to be a promising method of removing aflatoxin from food commodities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 43, Issue 2, February 2005, Pages 233-238
Journal: Food and Chemical Toxicology - Volume 43, Issue 2, February 2005, Pages 233-238
نویسندگان
A. Méndez-Albores, G. Arámbula-Villa, M.G.F. Loarca-Piña, E. Castaño-Tostado, E. Moreno-MartÃnez,