کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9034483 | 1562440 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CAR expression and inducibility of CYP2B genes in liver of rats treated with PB-like inducers
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کلمات کلیدی
TPDEMSA1,4-bis[2-(3,5-dichloropyridyloxy)]benzenePBREMCYP2BRXRTCPOBOPRetinoid X receptorNR1RT-PCRGAPDHelectrophoretic mobility shift assays - آزمایشات تحرک تحرک الکتروفورزOkadaic acid - اسید اوکادائیکdirect repeat - تکرار مستقیمbase pair - جفت پایهPhenobarbital - فنوباربیتالCAR - ماشینReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازconstitutive androstane receptor - گیرنده آندروستان پایدار
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The expression of the CAR gene and inducibility of CYP2B protein in the liver of male Wistar rats treated with phenobarbital (PB) and triphenyldioxane (TPD) were investigated. To clarify the role of phosphorylation/dephosphorylation in these processes, rats were treated with inhibitors of Ca2+/calmodulin-dependent kinase II (W7) or protein phosphatases PP1 and PP2A (OA) before induction. Constitutive expression of the CAR gene in livers of untreated rats was detected by multiplex RT-PCR. Treatment with W7 resulted in a 2.8-fold induction of CAR gene expression, whereas OA led to a 2.4-fold decrease of the mRNA level. The same results were obtained for CYP2B genes expression, which were increased by W7 treatment (two-fold) and decreased by OA (2.3-fold). PB-induction did not lead to significant alteration in the level of CAR gene expression, although CYP2B genes expression was enhanced two-fold over control values. TPD caused a two-fold increase of both CAR and CYP2B mRNA levels. Both inducers reduced the effects of inhibitors on CAR gene expression. Results of EMSA showed that PB, TPD or W7 alone induced formation of complexes of NR1 with nuclear proteins. Appearance of the complexes correlated with an increase in CYP2B expression, and their intensities were modulated by the protein kinase inhibitors. Thus, our results demonstrate that constitutive expressions of CAR as well as CYP2B during induction are regulated by phosphorylation/dephosphorylation processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 216, Issues 2â3, 15 December 2005, Pages 147-153
Journal: Toxicology - Volume 216, Issues 2â3, 15 December 2005, Pages 147-153
نویسندگان
Vladimir O. Pustylnyak, Lyudmila F. Gulyaeva, Vyacheslav V. Lyakhovich,