کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9034700 | 1132636 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glutathione metabolism is impaired in vitro by thallium(III) hydroxide
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کلمات کلیدی
GSHGPXGSSGDTNBEbselenDCI2-phenyl-1,2-benzisoselenazol-3(2H)-one5,5′-dithio-bis(2-nitrobenzoic acid) - 5،5'-dithio-bis (2-nitrobenzoic acid)SOD - سدToxicity - سمی بودنSuperoxide dismutase - سوکسوکس دیسموتازreduced glutathione - کاهش گلوتاتیونGlutathione - گلوتاتیونglutathione disulfide - گلوتاتیون دی سولفیدglutathione reductase - گلوتاتیون ردوکتازglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
The possibility that Tl(OH)3, the main Tl3+ specie present in water solutions, could interfere with the normal functioning of the glutathione-dependent antioxidant defense system was investigated. For this purpose, we used both the purified components of this system and rat brain cytosolic fractions. Tl(OH)3 (1-25 μM) significantly decreased the content of reduced glutathione (GSH) in both experimental systems, caused by GSH oxidation. In the same range of concentrations Tl(OH)3 inhibited glutathione peroxidase (GPx) activity in both models, using cumene hydroperoxide as the substrate. No alterations in the capacity of GPx activity to metabolize H2O2 were observed. Both in purified GR as well as in the cytosolic fraction, Tl(OH)3 (1-5 μM) inhibited GR activity, with a partial recovery of the activity at higher concentrations. While Tl(OH)3 inhibited the GR diaphorase activity of purified GR, in a concentration (1-25 μM) dependent manner, this effect was only observed in the cytosolic fractions at the highest concentration assessed (25 μM). Results indicate that, similarly to previous findings for Tl+ and Tl3+, Tl(OH)3 also alters the glutathione-dependent antioxidant defense system. The observed alterations of this important antioxidant protective pathway by the major Tl3+ specie in water solutions could be one mechanism involved in the oxidative stress associated to Tl-intoxication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 207, Issue 3, 28 February 2005, Pages 501-510
Journal: Toxicology - Volume 207, Issue 3, 28 February 2005, Pages 501-510
نویسندگان
Cecilia E. Hanzel, Marcela S. Villaverde, Sandra V. Verstraeten,