کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9034742 | 1132641 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of cultured, precision-cut rat liver slices as a model to study drug-induced liver apoptosis
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کلمات کلیدی
PBSCLVFCSHBSSHsc70TAA3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide - 3- [4،5-dimethylthiazol-2-yl] -2،5-diphenyltetrazolium bromideMTT - MTTALAT - TOOLSaspartate amino-transferase - آسپارتات آمین ترانسفرازalanine amino-transferase - آلانین آمینوترانسفرازAsat - اساتHistology - بافت شناسیRat liver slices - برش کبدی موشThioacetamide - تیوات آمیدApoptosis - خزان یاختهایintraperitoneal - داخل صفاقیfetal calf serum - سرم گوساله جنینPhosphate buffered saline - فسفات بافر شورPortal vein - ورن پورتال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of cultured, precision-cut rat liver slices as a model to study drug-induced liver apoptosis Evaluation of cultured, precision-cut rat liver slices as a model to study drug-induced liver apoptosis](/preview/png/9034742.png)
چکیده انگلیسی
The precision-cut liver slice culture model has been used widely to investigate drug metabolism and drug-induced necrosis. However, apoptosis, a key mediator of liver toxicity remains to be studied in this model. We evaluated apoptosis induced by thioacetamide (TAA) in rat liver slices, and in livers taken from TAA-treated rats as a control. Rat liver slices were treated with 50, 75 and 100Â mM of TAA for 15Â h. Histopathological examination of the liver slices revealed specific centrilobular localization of apoptotic hepatocytes at 75Â mM but randomly distributed at 100Â mM. Apoptosis in centrilobular hepatocytes was confirmed by appearance of cleavage products of caspase-3 and DNA fragmentation studied by TUNEL method. A concentration-dependent release of cytochrome c was observed in the slices, suggesting a role for mitochondria in the apoptosis triggered by TAA. The in vitro results were compared to the data obtained in male Sprague-Dawley rats given a single ip injection of 40Â mg/kg TAA and sacrificed 1, 2, 3 and 6Â h after dosing. Histopathological analyses showed specific centrilobular localization of apoptosis after 6Â h treatment. Caspase-3 activation, DNA fragmentation and cytochrome c release were also observed in the liver of rats treated with TAA. Overall these data indicated that precision-cut liver slices provide a valuable in vitro system to study drug-induced liver apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 207, Issue 2, 14 February 2005, Pages 203-214
Journal: Toxicology - Volume 207, Issue 2, 14 February 2005, Pages 203-214
نویسندگان
Valérie Moronvalle-Halley, Béatrice Sacré-Salem, Valérie Sallez, Gilles Labbe, Jean-Charles Gautier,