کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9037049 | 1133396 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nonylphenol-induced Ca2+ elevation and Ca2+-independent cell death in human osteosarcoma cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nonylphenol-induced Ca2+ elevation and Ca2+-independent cell death in human osteosarcoma cells Nonylphenol-induced Ca2+ elevation and Ca2+-independent cell death in human osteosarcoma cells](/preview/png/9037049.png)
چکیده انگلیسی
The effect of the environmental toxicant nonylphenol on cytosolic free Ca2+ concentration ([Ca2+]i) and proliferation has not been explored in human osteoblast-like cells. This study examined whether nonylphenol alters Ca2+ levels and causes cell death in MG63 human osteosarcoma cells. [Ca2+]i and cell death were measured using the fluorescent dyes fura-2 and WST-1 respectively. Nonylphenol at concentrations above 3 μM increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was reduced by 90% by removing extracellular Ca2+. The nonylphenol-induced Ca2+ influx was insensitive to blockade of L-type Ca2+ channel blockers. After pretreatment with 10 μM nonylphenol, 1 μM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) failed to induce [Ca2+]i rises. Inhibition of phospholipase C with 2 μM U73122 did not change nonylphenol-induced [Ca2+]i rises. The nonylphenol-induced [Ca2+]i rises were enhanced or inhibited by phorbol myristate acetate or GF 109203X, respectively. At concentrations of 10 and 20 μM nonylphenol killed 55% and 100% cells, respectively. The cytotoxic effect of 10 μM nonylphenol was unaltered by pre-chelating cytosolic Ca2+ with BAPTA. Collectively, in MG63 cells, nonylphenol induced [Ca2+]i rises by causing Ca2+ release from intracellular stores and Ca2+ influx from extracellular space. Furthermore, nonylphenol can cause Ca2+-unrelated cytotoxicity in a concentration-dependent manner.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 160, Issue 1, 30 December 2005, Pages 76-83
Journal: Toxicology Letters - Volume 160, Issue 1, 30 December 2005, Pages 76-83
نویسندگان
Jue-Long Wang, Chung-Shin Liu, Ko-Long Lin, Chiang-Ting Chou, Ching-Hong Hsieh, Chih-Hung Chang, Wei-Chuan Chen, Shiuh-Inn Liu, Shu-Shong Hsu, Hong-Tai Chang, Chung-Ren Jan,