کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038903 | 1133876 | 2005 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of a rapid in vitro androgen receptor transcriptional activation assay using AR-EcoScreen⢠cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of a rapid in vitro androgen receptor transcriptional activation assay using AR-EcoScreen⢠cells Evaluation of a rapid in vitro androgen receptor transcriptional activation assay using AR-EcoScreen⢠cells](/preview/png/9038903.png)
چکیده انگلیسی
An accurate and reliable in vitro assay system has been needed for first tier screening of endocrine disrupting chemicals. For the purpose, we previously developed stable AR-EcoScreen⢠cell lines to assess androgen receptor (AR)-mediated transcriptional activation. In this report, we evaluated AR-EcoScreen⢠cell lines as the phase I of prevalidation study by determining the intra-laboratory reproducibility, assay stability, and overall protocol performance of AR-EcoScreen⢠assays. Forty compounds recommended by the ICCVAM were tested for AR agonist and antagonist activity. The mean coefficient of variation (CV) for intra-assay reproducibility in the AR agonist assay was 4.35% for 5α-dihydrotestosterone (DHT), and that for the antagonist assay was 5.51% for hydroxyflutamide. The detection limit of the agonist assay was 2.3 Ã 10â11 M for 5α-dihydrotestosterone. Furthermore, we examined the overall performance of the method by comparing the predicted result with the ICCVAM classification. Thus, the overall sensitivity, specificity, and accuracy of the agonist assay were 89%, 94%, and 91%, respectively. For the antagonist assay, these values were 94%, 100%, and 96%, respectively. In summary, we concluded that AR-EcoScreen⢠method was ready to proceed to the phase II prevalidation study to asses the inter-laboratory variability and transfer of the protocol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 3, April 2005, Pages 335-352
Journal: Toxicology in Vitro - Volume 19, Issue 3, April 2005, Pages 335-352
نویسندگان
Naohiro Araki, Ken Ohno, Masahiro Takeyoshi, Mitsuru Iida,