کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9121457 1159187 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new soluble 10 kDa monoheme cytochrome c-552 from the anammox bacterium Candidatus “Kuenenia stuttgartiensis”
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
A new soluble 10 kDa monoheme cytochrome c-552 from the anammox bacterium Candidatus “Kuenenia stuttgartiensis”
چکیده انگلیسی
The chemolithoautotrophic anammox bacterium Candidatus “Kuenenia stuttgartiensis” grows anaerobically using ammonium as electron donor for nitrite reduction. More than 10% of the proteins in cell extracts of “K. stuttgartiensis” consist of c-type heme proteins. A 10 kDa soluble cytochrome c was purified from cell extracts using ultracentrifugation and anion exchange chromatography. The UV/Vis spectrum of the reduced cytochrome showed the γ, β and α absorption maxima at 419, 522 and 552 nm, respectively. The N-terminal amino acid sequence and peptide fragments of the tryptic digest of the protein were used to identify the corresponding gene. Analysis of the gene product showed that the protein was preceded by a 30 amino acids long leader sequence and that it belonged to the low-spin class ID cytochrome c. The CXXCH motive was located at the N-terminal site of the protein. The gene organization of the cytochrome showed some resemblance to cytochrome c clusters of unknown function in the genome of Nitrosomonas europaea and Geobacter sulfurreducens PCA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 252, Issue 2, 15 November 2005, Pages 273-278
نویسندگان
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