کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126951 | 1569967 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional mapping of the bovine Doppel gene promoter region
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کلمات کلیدی
βgalUTRkilobase(s) or 1000 bpPrion genePRNPDPLSV40PrPkDaMCsDMEMSDSORFFBSCATDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccokiloDalton(s) - kiloDalton (s)CAT assay - آزمون CATCm acetyltransferase - استیل ترانسفراز Cmβ-galactosidase - بتا گالاکتوزیدازEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوPrimer extension - فرمت اولیهopen reading frame - قاب خواندن بازRace - مسابقهuntranslated region(s) - ناحیه (های) غیر مترادفnucleotide(s) - نوکلئوتید (ها)Simian virus 40 - ویروس سیمانی 40Prion protein - پروتئین پریونmultiple cloning site(s) - چندین سایت شبیه سازی شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional mapping of the bovine Doppel gene promoter region Functional mapping of the bovine Doppel gene promoter region](/preview/png/9126951.png)
چکیده انگلیسی
The PRND gene encodes Doppel (Dpl), a protein that is strongly expressed in testis and at much lower levels in other tissues. Despite the recent discovery of Dpl involvement in spermiogenesis and in apoptotic death of cerebellar neurons, respectively in wild type and transgenic mice, the physiological role of this prion-like protein remains unknown. To better understand which factors may contribute to the modulation of PRND activity, a study of the bovine promoter region was performed. First, the transcription start site of PRND mRNA was identified using an innovative fluorescently labelled oligonucleotide extension (FLOE) method. The initiation site mapped 129 nt upstream of the protein coding sequence and represents a refinement of a previous assignment based on RACE. Second, deletion mutants of the 4530 nt encompassing 2704 nt 5â² of the bovine PRND, exon 1, intron 1, and the first 6 nt of exon 2, have been investigated with CAT-reporter assays in order to identify critical elements for the activation of the gene. The results showed that the region â 323/+ 32 (+ 1 is the transcription start site mapped by FLOE) represents the promoter region and contains positive cis-acting elements (CCAAT and E box) confirming previous reports with the mouse gene. Additional regulatory elements, including binding sites for repressor molecules, have been identified upstream of that region and in the first portion of intron 1, suggesting a complex tissue-specific regulation of Doppel gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 356, 15 August 2005, Pages 101-108
Journal: Gene - Volume 356, 15 August 2005, Pages 101-108
نویسندگان
Igor Del Vecchio, Alberto Azzalin, Elena Guidi, Giuseppe Amati, Tiziana Caramori, Cristina Uboldi, Sergio Comincini, Luca Ferretti,