کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127351 | 1569976 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the promoter of 1A6/DRIM, a novel cancer-related gene and identification of its transcriptional activator
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کلمات کلیدی
HLHEMSAUSFSV40PMSFDTTFBSElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزEST - استchromatin immunoprecipitation - ایمن سازی کروماتینTranscriptional regulation - تنظیم ترانزیتیTumorigenesis - تومورزاییExpressed Sequence Tag - تکرار اظهار نظرE-box - جعبه Edithiothreitol - دیتیوتریتولfetal bovine serum - سرم جنین گاوUpstream stimulatory factor - فاکتور تحرک بالاfluorescent in situ hybridization - فلورسنت در محل hybridizationphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدFish - ماهیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازSimian virus 40 - ویروس سیمانی 40CHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
1A6/DRIM (Down-regulated in Metastasis) has been reported to express at a high level in the gastric cancer tissues and the premalignant lesions implicating the involvement of 1A6/DRIM in cell transformation. Although the information regarding the putative functions and distribution of the 1A6/DRIM in different tissues and cell lines has been increasing recently, its promoter and promoter-regulating factors remain unknown. In this study, the transcription initiation site of 1A6/DRIM was confirmed to be located at 147 bp upstream of the ATG codon using the primer extension analysis. The minimal promoter region of the 1A6/DRIM is located between â47 and +42 of the transcription initiation site measured by luciferase reporter assays using a set of deletion constructs. In addition, an E-box is shown to be an essential element for transcriptional regulation of 1A6/DRIM demonstrated by luciferase assay with different deletion and mutation constructs. Finally, a transcription factor, upstream stimulatory factor 2 (USF2) was found to be an activator of the 1A6/DRIM through binding to the E-box demonstrated by luciferase reporter assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation (ChIP) assay. The structural analysis of the 1A6/DRIM promoter and the identification of its potential regulatory effecter may help us to understand its biological functions in regulating cancer development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 344, 3 January 2005, Pages 161-169
Journal: Gene - Volume 344, 3 January 2005, Pages 161-169
نویسندگان
Xiaoyan Xing, Xiaojuan Du, Zheming Lu, Tao Ning, Xiulan Su, Yang Ke,