کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140047 | 1570661 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells
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کلمات کلیدی
DICRab GTPaseGEFEntamoeba histolytica - Entamoeba هیستولیتیکاGTPase Activating Protein - GTPase فعال کردن پروتئینactin - اکتین Cho - برایCell motility - تحرک سلولیSecretion - ترشح Room temperature - دمای اتاقChinese hamster ovary cells - سلول های تخمدان هامستر چینیGAP - شکافguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینPolarity - قطبیhemagglutinin - هماگلوتینینdifferential interference contrast - کنتراست تداخل دیفرانسیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells A unique Rab GTPase, EhRabA, is involved in motility and polarization of Entamoeba histolytica cells](/preview/png/9140047.png)
چکیده انگلیسی
Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Motility, which requires cell polarization, is important to the virulence of this pathogen, as it may result in destruction of host tissues and invasion. To gain insight into these processes in Entamoeba, a unique Rab GTPase, EhRabA, which localizes to the leading edge of cells, was characterized. Cell lines expressing a dominant negative version of EhRabA (EhRabA-DN) were generated. These mutant cells exhibited alterations in cell shape, polarity, and motility, supporting a role for this Rab in the regulation of these processes. Consistent with the notion that a dynamic actin cytoskeleton is crucial to cell polarity and motility, these mutants also exhibited alterations in the actin cytoskeleton. Cells expressing EhRabA-DN also displayed defects in several virulence functions including the ability to adhere to host cells, destroy host cells, and release cysteine proteases. Mislocalization of a prominent adhesion molecule, the galactose/N-acetylgalactosamine (Gal/GalNAc) adherence lectin and reorganization of ordered lipid domains, known as lipid rafts, also accompanied expression of EhRabA-DN. Interestingly, several endocytic processes were unaffected by expression of EhRabA-DN. Together, these data suggest that EhRabA may be involved in the regulation of polarization, motility and actin cytoskeletal dynamics: functions that participate in the pathogenicity of Entamoeba.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 2, April 2005, Pages 161-173
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 2, April 2005, Pages 161-173
نویسندگان
Brenda H. Welter, Rhonda R. Powell, Megan Leo, Christine M. Smith, Lesly A. Temesvari,