کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140142 | 1163398 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trypanosoma cruzi histone H1 is phosphorylated in a typical cyclin dependent kinase site accordingly to the cell cycle
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کلمات کلیدی
CDKAUT-PAGEESI-TOF-MSa.m.u.CDKs - CDK هاm/z - m / zOkadaic acid - اسید اوکادائیکTrypanosoma cruzi - تریپانوزوم کروزیPhosphatase - فسفاتازPhosphorylation - فسفریلاسیونmass to charge ratio - نسبت جرم به شارژHydroxyurea - هیدروکسی اورهHistone H1 - هیستون H1atomic mass unit - واحد جرم اتمیCell cycle - چرخه سلولیcyclin dependent kinases - کیناز وابسته به سیکلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Histone H1 of most eukaryotes is phosphorylated during the cell cycle progression and seems to play a role in the regulation of chromatin structure, affecting replication and chromosome condensation. In trypanosomatids, histone H1 lacks the globular domain and is shorter when compared with the histone of other eukaryotes. We have previously shown that in Trypanosoma cruzi, the agent of Chagas' disease, histone H1 is phosphorylated and this increases its dissociation from chromatin. Here, we demonstrate using mass spectrometry analysis that T. cruzi histone H1 is only phosphorylated at the serine 12 in the sequence SPKK, a typical cyclin-dependent kinase site. We also found a correlation between the phosphorylation state of histone H1 and the cell cycle. Hydroxyurea and lactacystin, which, respectively, arrest parasites at the G1/S and G2/M stages of the cell cycle, increased the level of histone H1 phosphorylation. Cyclin-dependent kinase-related enzymes TzCRK3, and less intensely the TzCRK1 were able to phosphorylate histone H1 in vitro. Histone H1 dephosphorylation was prevented by treating the parasites with okadaic acid but not with calyculin A. These findings suggest that T. cruzi histone H1 phosphorylation is promoted by cyclin dependent kinases, present during S through G2 phase of the cell cycle, and its dephosphorylation is promoted by specific phosphatases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 1, March 2005, Pages 75-86
Journal: Molecular and Biochemical Parasitology - Volume 140, Issue 1, March 2005, Pages 75-86
نویسندگان
Julia Pinheiro Chagas da Cunha, Ernesto S. Nakayasu, Maria Carolina Elias, Daniel C. Pimenta, Maria Teresa Tellez-Inon, Federico Rojas, Muñoz Manuel, Igor C. Almeida, Sergio Schenkman,