کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9141907 | 1163886 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subtle sequence differences in a tumour-associated peptide epitope translate into major changes in antigenicity
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کلمات کلیدی
NOECDRNOESYscFvTOCSYVNTRnuclear magnetic resonance - رزونانس مغناطیسی هستهایNuclear Overhauser Effect SpectroscopY - Spectroscopy اثر Overhauser هسته ایAntigenicity - آنتی ژنیکnuclear overhauser effect - اثر بیش از حد هسته ایEpitope - اپیتوپNMR - تشدید مغناطیسی هستهای Variable number of tandem repeats - تعداد متغیر تکرارهای tandemtotal correlated spectroscopy - طیف سنجی همبستگی کلcomplementarity determining region - منطقه تعریف مکملPhage display - نمایش فاژیPeptide - پپتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
Antigenicity, the ability to bind to members of repertoire of diverse immune receptors, is a concept that is poorly characterised with respect to its defining parameters. To learn more about its makeup, we have investigated the ability of two peptides with highly related sequences, derived from the tumour-associated antigen mucin-1, to recruit in vitro members from a large naïve repertoire of synthetic human antibody fragments. One of the peptides represents the epitope that is immunodominant in mice. We now demonstrate that the other peptide, which differs from the first only by a very conservative aspartate-threonine to glutamate-serine change, is much less antigenic than the first peptide. This is so despite the fact that there is no observable difference in the tendency of the two peptides to adopt a structure in solution. Furthermore, the peptides differ in their immunodominant parts and the less antigenic peptide selects for antibody fragments targeting residues outside of the epitope considered to be immunodominant in mice. We conclude that subtle sequence changes greatly, affect antigenicity and immunodominance of epitopes in this important tumour-associated antigen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 42, Issue 11, July 2005, Pages 1321-1330
Journal: Molecular Immunology - Volume 42, Issue 11, July 2005, Pages 1321-1330
نویسندگان
Jonas Persson, Johan Lantto, Torbjörn Drakenberg, Mats Ohlin,