کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9194538 | 1580509 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The immunostimulatory activity of CpG oligonucleotides on microglial N9 cells is affected by a polyguanosine motif
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کلمات کلیدی
FCS4′,6′-Diamidino-2-phenylindole hydrochlorideOligodeoxynucleotidesCpG oligonucleotidesTLR9ODNPBSPFADAPIFITCfetal calf serum - سرم گوساله جنینphosphodiester - فسفودیدهاfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroglia - میکروگلیاهاparaformaldehyde - پارافرمالدهیدToll-like receptor 9 - گیرنده پولی مانند 9
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The immunostimulatory activity of CpG oligonucleotides on microglial N9 cells is affected by a polyguanosine motif The immunostimulatory activity of CpG oligonucleotides on microglial N9 cells is affected by a polyguanosine motif](/preview/png/9194538.png)
چکیده انگلیسی
Our data show that N9 cells express Toll-like receptor 9 (TLR9) and are activated by CpG-ODN, which leads to expression of interleukin-12p40 (IL12p40), tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS). A 3â²-end polyG motif inhibits phosphothioate (PS) CpG-ODN immunostimulatory activity but enhances the immunostimulatory activity of phosphodiester (PE) CpG-ODN. Correspondingly, a 3â²-end polyG motif improves the cellular uptake of PE CpG-ODN but does not change their cellular distribution pattern. Furthermore, PE CpG-ODN with a 3â²-end polyG motif interact with a much higher number of cellular proteins than PE CpG-ODN. These data indicate that the 3â²-end polyG motif could enhance the immunostimulatory activity of PE CpG-ODN in microglial N9 cells through increasing interaction with cellular proteins. Therefore PE CpG-ODN containing a 3â²-end polyG motif resulting in increased immunostimulatory activity might be promising alternate analogues for studies in the central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 161, Issues 1â2, April 2005, Pages 68-77
Journal: Journal of Neuroimmunology - Volume 161, Issues 1â2, April 2005, Pages 68-77
نویسندگان
Zhiren Zhang, Ketai Guo, Hermann J. Schluesener,