کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244067 | 1209899 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Negative Transcriptional Regulation of Human Colonic Smooth Muscle Cav1.2 Channels by p50 and p65 Subunits of Nuclear Factor-κB
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کلمات کلیدی
NF-κBEMSATNFPMSFSSCDTTSDSElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزACh - آهstandard saline citrate - استاندارد سدیم سیتراتAcetylcholine - استیل کولینbase pairs - جفت پایهdithiothreitol - دیتیوتریتولsodium dodecyl sulfate - سدیم دودسیل سولفاتresponse element - عنصر پاسخtumor necrosis factor - فاکتور نکروز تومورnuclear factor-κB - فاکتور هسته ای κBpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Negative Transcriptional Regulation of Human Colonic Smooth Muscle Cav1.2 Channels by p50 and p65 Subunits of Nuclear Factor-κB Negative Transcriptional Regulation of Human Colonic Smooth Muscle Cav1.2 Channels by p50 and p65 Subunits of Nuclear Factor-κB](/preview/png/9244067.png)
چکیده انگلیسی
Background & Aims: The expression of Cav1.2 channels in colonic circular smooth muscle cells and the contractility of these cells are suppressed in inflammation. Our aim was to investigate whether the activation of p50 and p65 nuclear factor-κB subunits mediates these effects. Methods: Primary cultures of human colonic circular smooth muscle cells and muscle strips were used. Results: The messenger RNA and protein expression of the pore-forming α1C subunit of Cav1.2 channels decreased time dependently in response to tumor necrosis factor α. This effect was blocked by prior transient transfection of the cells with antisense oligonucleotides to p50 or p65. The overexpression of p50 and p65 inhibited the constitutive expression of α1C. Three putative κB binding motifs were identified on the 5Ⲡflanking region of exon 1b of the human L-type calcium channel α1C gene. Progressive 5Ⲡdeletions of the promoter and point mutations of the κB binding motifs indicated that the two 5Ⲡbinding sites, but not the third 3Ⲡbinding site, were essential for the suppression of α1C. Transient transfection of human colonic circular muscle strips with antisense oligonucleotides to p50 and p65 decreased expression of the 2 nuclear factor-κB units and reversed the suppression of α1C, as well as that of the contractile response to acetylcholine, by 24 hours of treatment with tumor necrosis factor α. Conclusions: The activation of p50 and p65 by tumor necrosis factor α suppresses the expression of the α1C subunit of Cav1.2 channels in human colonic circular smooth muscle cells and their contractile response to acetylcholine. Nuclear factor-κB must bind concurrently to the two 5ⲠκB motifs on the promoter of α1C to produce this effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1518-1532
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1518-1532
نویسندگان
Xuan-Zheng Shi, Konrad Pazdrak, Nehad Saada, Bosong Dai, Philip Palade, Sushil K. Sarna,