کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9244078 | 1209899 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short Hairpin RNA Modulates Transforming Growth Factor β Signaling in Life-Threatening Liver Failure in Mice
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کلمات کلیدی
LPSTGF-β receptor ITGF-βRIITGF-β receptor IITGF-βALFshRNAeGFPRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهshort hairpin RNA - RNA موی سر کوتاهrecombinant human - انسان نوترکیبinterferon - اینترفرونIFN - اینترفرون هاTransforming growth factor β - تبدیل فاکتور رشد βTNF-α - فاکتور نکروز توموری آلفاlipopolysaccharide - لیپوپلی ساکاریدAcute liver failure - نارسایی حاد کبدیenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: Transforming growth factor β (TGF-β) receptor II (TGF-βRII), which is essential for TGF-β signaling and is involved in the causation or participates in the pathway of various human disorders, is consequently considered a key target for therapeutics and analysis of the pathophysiology associated with disruption of the TGF-β system. In the liver, TGF-β plays an essential role in hepatocyte apoptosis, growth inhibition, and progression of fibrogenesis. There is a critical need to introduce technology involving the TGF-β system, such as RNA interference (RNAi), which has high potential for in vivo therapeutics and analytical activities. Methods: Here, we investigated the effect of short hairpin RNA targeting TGF-βRII, using hepatocyte injury in human and mouse cell lines and liver injury mouse models. Results: We demonstrated that short hairpin RNA targeting TGF-βRII can be used to silence TGF-βRII genes in mouse and human cell lines, and physiologic and morphologic changes in hepatocytes suffering from acute injury are spared by RNAi-mediated gene silencing of the target gene and by suppressing downstream signal transduction. Furthermore, short hairpin RNA targeting TGF-βRII protected mice from life-threatening acute liver failure. Conclusions: Our study suggests the potential use of TGF-βRII silencing by RNAi as an analytical tool for TGF-β signaling and gene-specific therapy in human disorders.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1654-1662
Journal: Gastroenterology - Volume 129, Issue 5, November 2005, Pages 1654-1662
نویسندگان
Yoshiaki Mizuguchi, Shigeki Yokomuro, Takuya Mishima, Yasuo Arima, Tetsuya Shimizu, Yutaka Kawahigashi, Tomohiro Kanda, Hiroshi Yoshida, Toshihiro Takizawa, Takashi Tajiri,